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pSB74
(Plasmid #19591)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 19591 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pTrc99A
  • Backbone manufacturer
    E. Amann
  • Backbone size w/o insert (bp) 4176
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    thiosulfate reductase
  • Alt name
    phsABC
  • Species
    S. enterica serovar Typhimurium LT2
  • Insert Size (bp)
    5136
  • GenBank ID
    CP000026
  • Entrez Gene
    phsB

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer M13/pUC Reverse
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSB74 was a gift from Jay Keasling (Addgene plasmid # 19591 ; http://n2t.net/addgene:19591 ; RRID:Addgene_19591)
  • For your References section:

    Engineering hydrogen sulfide production and cadmium removal by expression of the thiosulfate reductase gene (phsABC) from Salmonella enterica serovar typhimurium in Escherichia coli. Bang SW, Clark DS, Keasling JD. Appl Environ Microbiol. 2000 Sep . 66(9):3939-44. 10.1128/AEM.66.9.3939-3944.2000 PubMed 10966412