|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||19619||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepDONR P2R-P3
Vector typeP4-P3 Donor vector
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Kanamycin
Copy numberHigh Copy
- Cloning method Gateway Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGC188 was a gift from Jane Hubbard (Addgene plasmid # 19619 ; http://n2t.net/addgene:19619 ; RRID:Addgene_19619)
For your References section:A "FLP-Out" system for controlled gene expression in Caenorhabditis elegans. Voutev R, Hubbard EJ. Genetics. 2008 Sep . 180(1):103-19. 10.1534/genetics.108.090274 PubMed 18723890