pHDR_G3BP1-V5-APEX2-mScarlet-I EF1a-Puro
(Plasmid
#196196)
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Purposehomologous recombination donor top insert V5-APEX2-mScarlet-I into the C-terminus of the human G3BP1 locus
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Depositing Lab
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Depositing OrganizationInstitut fuer Molekulare Biotechnologie (IMBA)
Located in Austria -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 196196 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 196196-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 196196-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepHDR_G3BP1-V5-APEX2-GFP
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Backbone manufacturerEugene Yeo; PubMed 29373831
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Vector typeHomology repair donor
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameG3BP1 homology arms flanking V5-APEX2-mScarlet-I and EF1a-puro selection cassette
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SpeciesH. sapiens (human)
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Entrez GeneG3BP1 (a.k.a. G3BP, HDH-VIII)
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Tag
/ Fusion Protein
- V5-APEX2-mScarlet-I (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
In Addgene vector 105284 the GFP was exchanged with the red fluorescent protein mScarlet-I using the plasmid pmScarlet-i_C1 (addgene 85044).
Please visit https://www.biorxiv.org/content/10.1101/2022.10.07.511280v2 for bioRxiv preprint.
DNA (Catalog # 196196-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 196196-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHDR_G3BP1-V5-APEX2-mScarlet-I EF1a-Puro was a gift from Jürgen Knoblich (Addgene plasmid # 196196 ; http://n2t.net/addgene:196196 ; RRID:Addgene_196196) -
For your References section:
Integrated transcriptome and proteome analysis reveals posttranscriptional regulation of ribosomal genes in human brain organoids. Sidhaye J, Trepte P, Sepke N, Novatchkova M, Schutzbier M, Durnberger G, Mechtler K, Knoblich JA. Elife. 2023 Mar 29;12:e85135. doi: 10.7554/eLife.85135. 10.7554/eLife.85135 PubMed 36989136
