pHDR_G3BP1-V5-APEX2-mScarlet-I EF1a-Puro
(Plasmid #196196)

• Purpose: homologous recombination donor top insert V5-APEX2-mScarlet-I into the C-terminus of the human G3BP1 locus
• Depositing Lab: Jürgen Knoblich
• Publication: Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.7554/eLife.85135.

Backbone

• Vector backbone: pHDR_G3BP1-V5-APEX2-GFP
• Backbone manufacturer: Eugene Yeo; PubMed 29373831
• Vector type: Homology repair donor
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: G3BP1 homology arms flanking V5-APEX2-mScarlet-I and EF1a-puro selection cassette
• Species: H. sapiens (human)
• Entrez Gene: G3BP1 (a.k.a. G3BP, HDH-VIII)
• Tag / Fusion Protein:
  • V5-APEX2-mScarlet-I (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• Supplemental Documents:
  • pHDR_G3BP1-V5-APEX2-mScarlet-I EF1a-Puro.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

In Addgene vector 105284 the GFP was exchanged with the red fluorescent protein mScarlet-I using the plasmid pmScarlet-i_C1 (addgene 85044).

Please visit https://www.biorxiv.org/content/10.1101/2022.10.07.511280v2 for bioRxiv preprint.