pcDNA3.1-3XHA-p53 Δ1-42
(Plasmid #196269)

• Purpose: Mammalian expression of 3xHA tagged p53 Δ1-42 (ΔTAD1)
• Depositing Lab: Laura Attardi
• Publication: Raj et al Mol Cell. 2022 Jul 7;82(13):2370-2384.e10. doi: 10.1016/j.molcel.2022.04.010. Epub 2022 May 4.

Backbone

• Vector backbone: pCDNA-3xHA
• Backbone size w/o insert (bp): 5500
• Total vector size (bp): 6547
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: p53 Δ1-42
• Species: M. musculus (mouse)
• Insert Size (bp): 1047
• Mutation: deleted amino acids 1 to 42
• Entrez Gene: Trp53 (a.k.a. Tp53, bbl, bfy, bhy, p44, p53)
• Promoter: CMV
• Tag / Fusion Protein:
  • 3xHA (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AscI (not destroyed)
• 3′ cloning site: PacI (not destroyed)
• 5′ sequencing primer: CMV/T7
• 3′ sequencing primer: bGH reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry