pGTVL2-BIRC2-BIR3
(Plasmid
#196367)
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Purposeprotein expression of the GST-tagged BIR3 domain
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 196367 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepGTVL2
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameBIRC2 BIR3 domain
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SpeciesH. sapiens (human)
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Mutationcontains only S260-Y352
- Promoter T7
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Tag
/ Fusion Protein
- His6-GST-TEV (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGTVL2-BIRC2-BIR3 was a gift from Stefan Knapp (Addgene plasmid # 196367 ; http://n2t.net/addgene:196367 ; RRID:Addgene_196367) -
For your References section:
A Toolbox for the Generation of Chemical Probes for Baculovirus IAP Repeat Containing Proteins. Schwalm MP, Berger LM, Meuter MN, Vasta JD, Corona CR, Rohm S, Berger BT, Farges F, Beinert SM, Preuss F, Morasch V, Rogov VV, Mathea S, Saxena K, Robers MB, Muller S, Knapp S. Front Cell Dev Biol. 2022 May 26;10:886537. doi: 10.3389/fcell.2022.886537. eCollection 2022. 10.3389/fcell.2022.886537 PubMed 35721509
