RCAS.Sfi Akt1-K179M
(Plasmid #196375)

• Purpose: Akt1 kinase negative mutant K179M in avian expression vector
• Depositing Lab: Peter Vogt
• Publication: Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(25):14950-5.

Backbone

• Vector backbone: RCAS(BP-A)
• Backbone size w/o insert (bp): 11600
• Modifications to backbone: SfiI sites added for directional cloning
• Vector type: Retroviral ; avian

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: c-akt1 K179M
• Alt name: kinase dead Akt1
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: CP Tsichlis's Lab/Fox Chase Cancer Center

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

https://doi.org/10.1016/0092-8674(95)90534-0