pCDF_TypeIC_target_sfGFP_invivo
(Plasmid
#196399)
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Purposeexpresses CRISPR Cascade Type IC target sequence for in vivo assay
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 196399 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCDF
- Total vector size (bp) 4255
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Streptomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nametype IC dsDNA target
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SpeciesSynthetic
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Insert Size (bp)36
- Promoter T7
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer taatacgactcactatagg (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDF_TypeIC_target_sfGFP_invivo was a gift from David Taylor (Addgene plasmid # 196399 ; http://n2t.net/addgene:196399 ; RRID:Addgene_196399) -
For your References section:
Structural snapshots of R-loop formation by a type I-C CRISPR Cascade. O'Brien RE, Bravo JPK, Ramos D, Hibshman GN, Wright JT, Taylor DW. Mol Cell. 2023 Mar 2;83(5):746-758.e5. doi: 10.1016/j.molcel.2023.01.024. Epub 2023 Feb 16. 10.1016/j.molcel.2023.01.024 PubMed 36805026
Map uploaded by the depositor.
