pET28b_TypeIC_acrIF2_invivo
(Plasmid
#196402)
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Purposeexpresses acrIF2 protein targeting CRISPR Cascade Type IC for in vivo assay
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 196402 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepET28b
- Total vector size (bp) 5457
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameanti-CRISPR IF2 protein
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Alt nameAcrIF2
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SpeciesP. aeruginosa
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Insert Size (bp)273
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GenBank IDUniProt: Q6TM72 WP_015972868.1
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Entrez Geneorf30 (a.k.a. D3112p30)
- Promoter T7
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer taatacgactcactatagg (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made bygene cloned from addgene plasmid #89234
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET28b_TypeIC_acrIF2_invivo was a gift from David Taylor (Addgene plasmid # 196402 ; http://n2t.net/addgene:196402 ; RRID:Addgene_196402) -
For your References section:
Structural snapshots of R-loop formation by a type I-C CRISPR Cascade. O'Brien RE, Bravo JPK, Ramos D, Hibshman GN, Wright JT, Taylor DW. Mol Cell. 2023 Mar 2;83(5):746-758.e5. doi: 10.1016/j.molcel.2023.01.024. Epub 2023 Feb 16. 10.1016/j.molcel.2023.01.024 PubMed 36805026
Map uploaded by the depositor.
