pcDNA3.1(+)-C-eGFP-Ctdnep1_D67EsiR
(Plasmid #196518)

• Purpose: Expression of Ctdnep1_D67E-GFP
• Depositing Lab: Edgar Gomes
• Publication: Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e8. doi: 10.1016/j.cub.2021.01.007. Epub 2021 Feb 9.

Backbone

• Vector backbone: pcDNA3.1+EeGFP
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Ctdnep1_D67EsiR
• Species: H. sapiens (human)
• Insert Size (bp): 799
• Mutation: D67E mutation (phosphastase dead). Silent mutations for siRNA resistance
• Entrez Gene: CTDNEP1 (a.k.a. DULLARD, HSA011916, NET56)
• Promoter: CMV promoter; T7 promoter
• Tag / Fusion Protein:
  • eGFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: NotI (unknown if destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Contains attb1 and attb2 sites and a Linker sequence between Ctdnep1 and eGFP