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BirA_BVES untagged
(Plasmid #196581)

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Item Catalog # Description Quantity Price (USD)
Plasmid 196581 Standard format: Plasmid sent in bacteria as agar stab 1 $94 *
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DNA 196581-D50 50 μg of DNA in Tris buffer $495 *
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DNA 196581-D100 100 μg of DNA in Tris buffer $585 *
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Backbone

  • Vector backbone
    pFBOH-LIC edited
  • Backbone manufacturer
    SGC (Addgene Plasmid #26099)
  • Vector type
    Baculovirus expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BirA_Ecoli
  • Species
    E. coli
  • Insert Size (bp)
    963

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

SGC ID: BirA_Ecoli:TOC025-E08:C244778

Please visit https://www.biorxiv.org/content/10.1101/2022.11.21.516512v1 for bioRxiv preprint.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BirA_BVES untagged was a gift from Cheryl Arrowsmith (Addgene plasmid # 196581 ; http://n2t.net/addgene:196581 ; RRID:Addgene_196581)

  • For your References section:

    Delineation of functional subdomains of Huntingtin protein and their interaction with HAP40. Alteen MG, Deme JC, Alvarez CP, Loppnau P, Hutchinson A, Seitova A, Chandrasekaran R, Silva Ramos E, Secker C, Alqazzaz M, Wanker EE, Lea SM, Arrowsmith CH, Harding RJ. Structure. 2023 Sep 7;31(9):1121-1131.e6. doi: 10.1016/j.str.2023.06.002. Epub 2023 Jun 29. 10.1016/j.str.2023.06.002 PubMed 37390814
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