pzk408-pmax-3xflag-dpspCas13b(AAAA)-NES
(Plasmid
#196828)
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PurposeExpress dpdpCas13b with AAAA mutation
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Depositing Lab
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Depositing OrganizationJackson Laboratory
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 196828 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 196828-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 196828-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepAC90-pmaxGW
- Backbone size w/o insert (bp) 2955
- Total vector size (bp) 6431
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert namedpspCas13b(AAAA)
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Insert Size (bp)3464
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MutationK367A, D369A and K370A
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Tags
/ Fusion Proteins
- 3XFLAG (N terminal on insert)
- NES (C terminal on insert)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2022.06.21.497089v1 for bioRxiv preprint.
DNA (Catalog # 196828-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 196828-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pzk408-pmax-3xflag-dpspCas13b(AAAA)-NES was a gift from Albert Cheng (Addgene plasmid # 196828 ; http://n2t.net/addgene:196828 ; RRID:Addgene_196828) -
For your References section:
Simultaneous multifunctional transcriptome engineering by CRISPR RNA scaffold. Liu Z, Jillette N, Robson P, Cheng AW. Nucleic Acids Res. 2023 Aug 11;51(14):e77. doi: 10.1093/nar/gkad547. 10.1093/nar/gkad547 PubMed 37395412
Map uploaded by the depositor.
