pCAG-lox-inv[Talpha1-iCre-pA]-lox-mNeonGreen-hCentrin1
(Plasmid #196890)

• Purpose: Neuron-specific expression of human (h)Centrin fused to mNeonGreen
• Depositing Lab: Frank Bradke
• Publication: Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16. doi: 10.1016/j.neuron.2023.01.020. Epub 2023 Feb 15.

Backbone

• Vector backbone: pCAG-lox
• Backbone size w/o insert (bp): 7557
• Vector type: Mammalian Expression, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mNeonGreen-hCentrin1
• Species: H. sapiens (human)
• Insert Size (bp): 1278
• Entrez Gene: CETN1 (a.k.a. CEN1, CETN)
• Promoter: Quimeric CAG

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: pCAG forward (5'-GCAACGTGCTGGTTATTGTG-3')
• 3′ sequencing primer: M13_ (-20) forward primer (5'-GTAAAACGACGGCCAGT-3')

Resource Information

• Supplemental Documents:
  • pCAG-lox-inv[Talpha1-iCre-pA]-lox-mNeonGreen-hCentrin1.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • mNeonGreen Label License
• Industry Terms:
  • Not Available to Industry