pmCherry-TdIF2
(Plasmid #196906)

• Purpose: visualization of TdIF2/ERBP as a fusion to mCherry
• Depositing Lab: Kenji Sugimoto
• Publication: Fukada et al Biosci Biotechnol Biochem. 2019 Jul;83(7):1255-1262. doi: 10.1080/09168451.2019.1591265. Epub 2019 Mar 24.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 6800
• Modifications to backbone: Our pmCherry C-1 was constructed by replacing Nhe I-Bgl II EGFP fragment with Nhe I-Bgl II 700bp mCherry DNA fragment, amplified by PCR using pRSET-B mCherry (provided by Prof. Roger Y. Tsien, University of California, San Diego) as a template. So, no AgeI site at 5’ nor BspE I site at 3’.
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: TdIF2
• Alt name: ERBP
• Alt name: AB_046574
• Species: H. sapiens (human)
• Insert Size (bp): 2100
• Promoter: CMV
• Tag / Fusion Protein:
  • mCherry (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Hind III (unknown if destroyed)
• 3′ cloning site: Sal I (unknown if destroyed)
• 5′ sequencing primer: EGFP side
• 3′ sequencing primer: SV40 poly A side

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Obtained by screening the human cDNA library (purchased from Origene). mCherry C-1 was amplified by PCR using pRSET-B mCherry (provided by Prof. Roger Y. Tsien, University of California, San Diego) as a template.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

2.1 kb PCR fragment of a pJG4-5 clone from OriGene human MCF7 cDNA library, was cloned the same sites of pEGFP-C1 and our original “pmCherry C-1” (without Eco47III, AgeI, BspEI sites).