pEGFP-FCHO1
(Plasmid
#196910)
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Purposevisualization of clathrin-coaed vesicle formation in living mammalian cells
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Depositing Lab
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Depositing OrganizationOsaka Metropolitan University
Located in Japan -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 196910 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 196910-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 196910-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepEGFP-C1
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Backbone manufacturerClontech
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameFCHO1
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Alt nameKIAA0290
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Alt nameFes/CIP4 homology (FCH) domain only 1
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SpeciesH. sapiens (human)
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Entrez GeneFCHO1 (a.k.a. IMD76)
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Tag
/ Fusion Protein
- EGFP (N terminal on insert)
Resource Information
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A portion of this plasmid was derived from a plasmid made byCloned gene (KIAA0290) was obtained from Kazusa (http://www.kazusa.or.jp/huge/).
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
DNA (Catalog # 196910-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 196910-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEGFP-FCHO1 was a gift from Kenji Sugimoto (Addgene plasmid # 196910 ; http://n2t.net/addgene:196910 ; RRID:Addgene_196910) -
For your References section:
Dynamic behavior of FCHO1 revealed by live-cell imaging microscopy: its possible involvement in clathrin-coated vesicle formation. Sakaushi S, Inoue K, Zushi H, Senda-Murata K, Fukada T, Oka S, Sugimoto K. Biosci Biotechnol Biochem. 2007 Jul;71(7):1764-8. doi: 10.1271/bbb.60720. Epub 2007 Jul 7. 10.1271/bbb.60720 PubMed 17617719
