pEGFP-FCHO1
(Plasmid
#196910)
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Purposevisualization of clathrin-coaed vesicle formation in living mammalian cells
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 196910 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepEGFP-C1
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Backbone manufacturerClontech
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameFCHO1
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Alt nameKIAA0290
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Alt nameFes/CIP4 homology (FCH) domain only 1
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SpeciesH. sapiens (human)
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Tag
/ Fusion Protein
- EGFP (N terminal on insert)
Resource Information
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A portion of this plasmid was derived from a plasmid made byCloned gene (KIAA0290) was obtained from Kazusa (http://www.kazusa.or.jp/huge/).
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEGFP-FCHO1 was a gift from Kenji Sugimoto (Addgene plasmid # 196910 ; http://n2t.net/addgene:196910 ; RRID:Addgene_196910) -
For your References section:
Dynamic behavior of FCHO1 revealed by live-cell imaging microscopy: its possible involvement in clathrin-coated vesicle formation. Sakaushi S, Inoue K, Zushi H, Senda-Murata K, Fukada T, Oka S, Sugimoto K. Biosci Biotechnol Biochem. 2007 Jul;71(7):1764-8. doi: 10.1271/bbb.60720. Epub 2007 Jul 7. 10.1271/bbb.60720 PubMed 17617719
