Atg24 1B
(Plasmid
#197046)
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PurposeHis6 tagged Atg24 from S. cerevisiae for expression in E. coli.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 197046 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepET His6 TEV LIC cloning vector (1B)
- Backbone size w/o insert (bp) 5340
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameAtg24
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Alt nameSNX4
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Alt nameCVT13
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Alt nameAtg24
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)1269
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GenBank IDNM_001181470
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Tag
/ Fusion Protein
- 6His-TEV (N terminal on backbone)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7 Promoter
- 3′ sequencing primer T7 Reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Atg24 1B was a gift from Michael Ragusa (Addgene plasmid # 197046 ; http://n2t.net/addgene:197046 ; RRID:Addgene_197046) -
For your References section:
A Comparative Analysis of the Membrane Binding and Remodeling Properties of Two Related Sorting Nexin Complexes Involved in Autophagy. Reinhart EF, Katzenell S, Andhare D, Bauer KM, Ragusa MJ. Biochemistry. 2022 Apr 14. doi: 10.1021/acs.biochem.2c00007. 10.1021/acs.biochem.2c00007 PubMed 35421303
Map uploaded by the depositor.
