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Addgene

pMAT_M10_PQQAT
(Plasmid #197093)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 197093 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pMAT
  • Backbone manufacturer
    Thermofisher
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    This plasmid contains the coding sequence for signaling peptide M10, which contains the PQQAT signaling motif.
  • Species
    H. sapiens (human)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    CD8a hinge and transmembrane domain: Provided by Michael Milone and Dr. Carl June UPENN.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMAT_M10_PQQAT was a gift from Wendell Lim (Addgene plasmid # 197093 ; http://n2t.net/addgene:197093 ; RRID:Addgene_197093)
  • For your References section:

    Decoding CAR T cell phenotype using combinatorial signaling motif libraries and machine learning. Daniels KG, Wang S, Simic MS, Bhargava HK, Capponi S, Tonai Y, Yu W, Bianco S, Lim WA. Science. 2022 Dec 16;378(6625):1194-1200. doi: 10.1126/science.abq0225. Epub 2022 Dec 8. 10.1126/science.abq0225 PubMed 36480602