pRK5-Myc-PSK2 (1-416)
(Plasmid #197123)

• Purpose: Expression of Myc-tagged PSK2 (aa1-416) / TAOK1 (aa1-416) in mammalian cells
• Depositing Lab: Jonathan Morris
• Depositing Organization: King's College London
  Located in the United Kingdom of Great Britain and Northern Ireland
• Publication: Tavares et al J Biol Chem. 2013 May 24;288(21):15418-29. doi: 10.1074/jbc.M112.448183. Epub 2013 Apr 12.

Backbone

• Vector backbone: pRK5
• Backbone manufacturer: Alan Hall
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 5951
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TAOK1 (aa1-416)
• Alt name: PSK2 (aa1-416)
• Alt name: KIAA1361 (aa1-416)
• Species: H. sapiens (human)
• Insert Size (bp): 1248
• Mutation: Deleted C-terminus
• GenBank ID: 57551
• Entrez Gene: TAOK1 (a.k.a. DDIB, KFC-B, MAP3K16, MARKK, PSK-2, PSK2, TAO1, hKFC-B, hTAOK1)
• Promoter: SV40
• Tag / Fusion Protein:
  • Myc (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamH1 (not destroyed)
• 3′ cloning site: EcoR1 (not destroyed)
• 5′ sequencing primer: GGTCCAACTGCACCTCGG
• 3′ sequencing primer: GCAATAAACAAGTTGGGC

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Cloned by Jonathan Morris at KCL (subject to MTA with Addgene).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

DNA (Catalog # 197123-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 197123-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.