pAAV-hPhyB621(Y276H)-P2A-m1V
(Plasmid
#197377)
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PurposeThe AAV vector for PhyB621(Y276H) and m1V
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 197377 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV
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Backbone manufacturerCell Biolabs Inc
- Backbone size w/o insert (bp) 4600
- Total vector size (bp) 7255
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePhytochrome B Y276H(1-621 aa)
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Alt namePhyB621(Y276H)
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SpeciesA. thaliana (mustard weed)
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Insert Size (bp)2631
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MutationHuman codon optimized PhyB (1-621 aa), Y276H mutation
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GenBank IDNM_001335612.1
- Promoter CMV
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Tag
/ Fusion Protein
- P2A-m1V (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byDr. Kazuhiro Aoki (Addgene plasmid # 100540: pCAGGS-hPhyBY276H-mCherry-HRasCT)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-hPhyB621(Y276H)-P2A-m1V was a gift from Michiyuki Matsuda (Addgene plasmid # 197377 ; http://n2t.net/addgene:197377 ; RRID:Addgene_197377)