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Addgene

pAAV-hPhyB621(Y276H)-P2A-m1V
(Plasmid #197377)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 197377 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pAAV
  • Backbone manufacturer
    Cell Biolabs Inc
  • Backbone size w/o insert (bp) 4600
  • Total vector size (bp) 7255
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Phytochrome B Y276H(1-621 aa)
  • Alt name
    PhyB621(Y276H)
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    2631
  • Mutation
    Human codon optimized PhyB (1-621 aa), Y276H mutation
  • GenBank ID
    NM_001335612.1
  • Promoter CMV
  • Tag / Fusion Protein
    • P2A-m1V (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. Kazuhiro Aoki (Addgene plasmid # 100540: pCAGGS-hPhyBY276H-mCherry-HRasCT)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-hPhyB621(Y276H)-P2A-m1V was a gift from Michiyuki Matsuda (Addgene plasmid # 197377 ; http://n2t.net/addgene:197377 ; RRID:Addgene_197377)