pCMV-T7-ABE8e-SpRY-HF1-P2A-EGFP (LM434)
(Plasmid
#197507)
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PurposeCMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpRY-HF1(D10A) and P2A-EGFP
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 197507 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCMV_ABEmax_P2A_GFP (Addgene Plasmid #112101)
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Vector typeMammalian Expression, CRISPR ; in vitro transcription; T7 promoter
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepCMV-T7-BPNLS-ABE8e-nSpRY-HF1-BPNLS-P2A-EGFP
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Alt nameLM434
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Alt nameABE8e-SpRY-HF1
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SpeciesSynthetic
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MutationABE8e mutations in TadA and nSpRY-HF1(D10A/A61R/N497A/R661A/Q695A/Q926A/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R)
- Promoter CMV and T7
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Tags
/ Fusion Proteins
- BPNLS (N terminal on insert)
- BPNLS-P2A-EGFP (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer oBK6928-CCAAGTCTCCACCCCATTGACG
- 3′ sequencing primer oBK219-GGGAGTGGCACCTTCCAGGGTC (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2023.01.20.524978v1 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-T7-ABE8e-SpRY-HF1-P2A-EGFP (LM434) was a gift from Benjamin Kleinstiver (Addgene plasmid # 197507 ; http://n2t.net/addgene:197507 ; RRID:Addgene_197507) -
For your References section:
Optimization of base editors for the functional correction of SMN2 as a treatment for spinal muscular atrophy. Alves CRR, Ha LL, Yaworski R, Sutton ER, Lazzarotto CR, Christie KA, Reilly A, Beauvais A, Doll RM, de la Cruz D, Maguire CA, Swoboda KJ, Tsai SQ, Kothary R, Kleinstiver BP. Nat Biomed Eng. 2023 Dec 6. doi: 10.1038/s41551-023-01132-z. 10.1038/s41551-023-01132-z PubMed 38057426
Map uploaded by the depositor.
