Myc-Nix-S212A
(Plasmid #197514)

• Purpose: Mammalian expression of myc-tagged human Nix with an alanine substitution of serine-212. Based on Myc-Nix (#100795)
• Depositing Lab: Joseph Gordon
• Publication: da Silva Rosa et al Autophagy. 2021 Sep;17(9):2257-2272. doi: 10.1080/15548627.2020.1821548. Epub 2020 Oct 12.

Backbone

• Vector backbone: pcDNA3.1
• Backbone size w/o insert (bp): 5400
• Total vector size (bp): 6060
• Modifications to backbone: Myc tag inserted in multiple cloning region
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: BCL2 interacting protein 3 like
• Alt name: BNIP3L (a.k.a. BNIP3a, NIX)
• Species: H. sapiens (human)
• Insert Size (bp): 660
• Mutation: Serine 212 changed to alanine
• GenBank ID: NM_004331.3
• Entrez Gene: BNIP3L (a.k.a. BNIP3a, NIP3L, NIX)
• Promoter: CMV
• Tag / Fusion Protein:
  • Myc (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry