TRF2ΔBΔM/pENTR
(Plasmid #19797)

• Depositing Lab: Mark Mattson
• Publication: van Steensel et al Cell. 1998 Feb 6. 92(3):401-13.

Backbone

• Vector backbone: pENTR/D-TOPO
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 2820
• Vector type: Entry Vector
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: TOP10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TRF2
• Alt name: TERF2
• Species: H. sapiens (human)
• Insert Size (bp): 1100
• Mutation: deleted amino acids 1-44 and 450-500
• GenBank ID: NM_005652.1 NP_005643.1
• Entrez Gene: TERF2 (a.k.a. TRBF2, TRF2)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: pENTR4-R
• 3′ sequencing primer: M13pUC-fwd

Resource Information

• A portion of this plasmid was derived from a plasmid made by: de Lange

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The missing codon is due to a reverse primer with a stop codon at 1474 of TRF2 gene: GAATTC TTA CTTCTGCTTTTTTGTTATATTGGTTGT. It generates a 50aa in frame deletion from c-terminal tail.