pSB_syb25
(Plasmid
#197992)
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PurposeExpressed sybody 25 as a C-terminal myc-His-tagged construct
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Depositing Lab
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Sequence Information
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Sequences (2) — Accept Affinity Reagent Sequence Policy
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Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 197992 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepSB_init
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSybody 25
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SpeciesSynthetic
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Insert Size (bp)441
- Promoter Arabinose
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Tag
/ Fusion Protein
- Myc-His (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BspQ1 (not destroyed)
- 3′ cloning site BspQ1 (not destroyed)
- 5′ sequencing primer pBAD_F
- 3′ sequencing primer pBAD_R (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSB_syb25 was a gift from Simon Newstead (Addgene plasmid # 197992 ; http://n2t.net/addgene:197992 ; RRID:Addgene_197992)