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Anti-HRAS [Y13-259]
(Antibody #198004)

Ordering

Item Catalog # Description Quantity Price (USD)
Recombinant Antibody 198004-rAb 100 µg of purified recombinant antibody $250 *
Recombinant Antibody trial size 198004-rAb.T 20 µg of purified recombinant antibody $85 *

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Features

Production & Usage

  • Antigen Plasmid
    mEGFP-HRas
  • Purification
    Purification from cell culture supernatant using affinity chromatography followed by buffer exchange with centrifugal columns.
  • Storage Buffer
    PBS + 1 mM sodium azide

Delivery

  • Concentration
    0.9–1.1 mg/mL
  • Storage
    Upon receipt, prepare single use aliquots and store at -20 °C until use. Avoid multiple freeze thaw cycles.
  • Shipment
    Blue ice or room temp

Resource Information

  • Persistent Resource Identifier
    RRID:AB_2928136

Terms and Licenses

Target Antigen

Protein GTPase HRas
Antigen Description Harvey murine sarcoma virus-transformed NRK cells
Species R. norvegicus (rat)
Gene Hras
Alternative Names
  • H-Ras-1
  • Transforming protein p21
  • c-H-ras
  • p21ras
External References

Applications (2)

Immunocytochemistry

1 image
Immunocytochemistry image by Addgene
Submitted By: Addgene
Antibody Type
Recombinant
Sample Species
H. sapiens (human)
Result
Pass

Western Blot

1 image
Western Blot image by Addgene
Submitted By: Addgene
Antibody Type
Recombinant
Sample Species
H. sapiens (human)
Result
Pass
How to cite this antibody ( Back to top)

These antibodies were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the antibodies were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Anti-HRAS [Y13-259] - from Melina Fan (Addgene antibody # 198004 ; http://n2t.net/addgene:198004 ; RRID:AB_2928136)
  • For your References section:

    Monoclonal antibodies to the p21 products of the transforming gene of Harvey murine sarcoma virus and of the cellular ras gene family. Furth ME, Davis LJ, Fleurdelys B, Scolnick EM. J Virol. 1982 Jul;43(1):294-304. doi: 10.1128/JVI.43.1.294-304.1982. 10.1128/JVI.43.1.294-304.1982 PubMed 6287003