pACT2-μ3A
(Plasmid #198174)

• Purpose: Expression of GAL4 transcriptional activation domain (AD)-AP-3 μ3A fusion protein in yeast (yeast two-hybrid assays)
• Depositing Lab: Juan Bonifacino
• Publication: Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi: 10.1016/j.devcel.2013.10.006.

Backbone

• Vector backbone: pACT2
• Backbone size w/o insert (bp): 8117
• Total vector size (bp): 9373
• Vector type: Yeast Expression
• Selectable markers: LEU2

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: AP-3 μ3A
• Species: R. norvegicus (rat)
• Insert Size (bp): 1265
• Mutation: silent substitution in codon 225
• Promoter: ADH1
• Tags / Fusion Proteins:
  • GAL4 transcriptional activation domain (AD) fragment (N terminal on backbone)
  • HA tag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamH1 (not destroyed)
• 3′ cloning site: XhoI (destroyed during cloning)
• 5′ sequencing primer: GAL4 transcriptional activation domain (AD) forward
• 3′ sequencing primer: GAL4 transcriptional activation domain (AD) reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry