Dyn1 R271E E720R
(Plasmid
#198344)
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PurposeMammalian expression plasmid of GFP-chimera proteins.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 198344 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepEGFP-N1
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Backbone manufacturerClontech
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDynamin1
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SpeciesH. sapiens (human)
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MutationR271E D291R
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Entrez GeneDNM1 (a.k.a. DEE31, DNM, EIEE31)
- Promoter CMV
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Tag
/ Fusion Protein
- EGFP (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CNV-F
- 3′ sequencing primer SV40-pArev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Dyn1 R271E E720R was a gift from Justin Taraska (Addgene plasmid # 198344 ; http://n2t.net/addgene:198344 ; RRID:Addgene_198344) -
For your References section:
Cryo-EM structures of membrane-bound dynamin in a post-hydrolysis state primed for membrane fission. Jimah JR, Kundu N, Stanton AE, Sochacki KA, Canagarajah B, Chan L, Strub M, Wang H, Taraska JW, Hinshaw JE. Developmental Cell 2024 10.1016/j.devcel.2024.04.008