Skip to main content
Addgene
Open Menu

pEGFP-Aurora A
(Plasmid #198402)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 198402 Standard format: Plasmid sent in bacteria as agar stab 1 $94
Add to Cart
DNA 198402-D50 50 μg of DNA in Tris buffer $495
Add to Cart
DNA 198402-D100 100 μg of DNA in Tris buffer $585
Add to Cart

Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clonetech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 5900
  • Modifications to backbone
    EcoR I and Sal I sites of pEGFP-ΔC2, which had been derived from pEGFP-C1 by cutting and filling-in Hind III site and converted to C2 reading frame
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Aurora A
  • Alt name
    BC_001280
  • gRNA/shRNA sequence
    cDNA
  • Species
    H. sapiens (human)
  • Tag / Fusion Protein
    • EGFP (N terminal on insert)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. Urano, Nagoya Univ.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pEGFP-Aurora-A was constructed by inserting a 1.2 kb fragment of human Aurora-A cDNA into the BglII and SalI site of pEGFP- C1 (Clontech, Palo Alto, CA, USA).

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-Aurora A was a gift from Kenji Sugimoto (Addgene plasmid # 198402 ; http://n2t.net/addgene:198402 ; RRID:Addgene_198402)

  • For your References section:

    Molecular dynamics of Aurora-A kinase in living mitotic cells simultaneously visualized with histone H3 and nuclear membrane protein importinalpha. Sugimoto K, Urano T, Zushi H, Inoue K, Tasaka H, Tachibana M, Dotsu M. Cell Struct Funct. 2002 Dec;27(6):457-67. PubMed 12576638
Related items:
Commonly requested with: