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pRevTRE MKL1 S449A/T450A/S454A
(Plasmid #19847)

Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 19847 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
    pRev TRE
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 6500
  • Vector type
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy


  • Gene/Insert name
    MKL1 S449A/T450A/S454A
  • Alt name
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    changed Serine 449, Threonine 450 and Serine 454 to Alanines.
  • Entrez Gene
    MRTFA (a.k.a. BSAC, MAL, MKL, MKL1, MRTF-A)
  • Tag / Fusion Protein
    • 3xFLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site Sal I (not destroyed)
  • 5′ sequencing primer NA
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

The 3xFlag in the vector is from Sigma, p3xFlag-CMV-7.1 has variant flag sequences (DYKDHD) and is recognized well by their anti-flag monoclonal M2.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRevTRE MKL1 S449A/T450A/S454A was a gift from Ron Prywes (Addgene plasmid # 19847 ; ; RRID:Addgene_19847)
  • For your References section:

    Serum-induced phosphorylation of the SRF coactivator MKL1 by the ERK1/2 pathway inhibits its nuclear localization. Muehlich S, Wang R, Lee SM, Lewis TC, Dai C, Prywes R. Mol Cell Biol. 2008 Aug 11. ():. 10.1128/MCB.00427-08 PubMed 18694962