Lenti-guide-puro mRab12-1
(Plasmid #198475)

• Purpose: lentiviral stable expression of mRab12 gRNA 1
• Depositing Lab: Suzanne Pfeffer
• Publication: Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.7554/eLife.87098.

Backbone

• Vector backbone: lenti-guide puro
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mouse Rab12
• gRNA/shRNA sequence: CCAGCTTATTTCCAACCAGG
• Species: M. musculus (mouse)
• Insert Size (bp): 20
• Entrez Gene: Rab12 (a.k.a. 2900054P15Rik)
• Promoter: U6

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)
• 5′ sequencing primer: gcctatttcccatgattccttc

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2023.02.17.529028v1 for bioRxiv preprint.