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(Plasmid #198525)


Item Catalog # Description Quantity Price (USD)
Plasmid 198525 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 5524
  • Total vector size (bp) 7671
  • Vector type
    Yeast Expression
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Entrez Gene
    HOOK3 (a.k.a. HK3)
  • Promoter ADH1
  • Tag / Fusion Protein
    • GAL4-DNA binding domain (BD) fragment (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer GAL4 DNA binding domain forward
  • 3′ sequencing primer GAL4 DNA binding domain reverse
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGBT9-Hook3 was a gift from Juan Bonifacino (Addgene plasmid # 198525 ; ; RRID:Addgene_198525)
  • For your References section:

    The FTS-Hook-FHIP (FHF) complex interacts with AP-4 to mediate perinuclear distribution of AP-4 and its cargo ATG9A. Mattera R, Williamson CD, Ren X, Bonifacino JS. Mol Biol Cell. 2020 Apr 15;31(9):963-979. doi: 10.1091/mbc.E19-11-0658. Epub 2020 Feb 19. 10.1091/mbc.E19-11-0658 PubMed 32073997