|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||19872||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5862
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameEukaryotic translation initiation factor 2C, 2
Alt nameEIF2C2; Q10; AGO2; MGC3183
SpeciesH. sapiens (human)
Insert Size (bp)2580
Entrez GeneAGO2 (a.k.a. CASC7, EIF2C2, LINC00980, PPD, Q10)
/ Fusion Protein
- MYC (N terminal on backbone)
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pDESTmycEIF2C2 was a gift from Thomas Tuschl (Addgene plasmid # 19872 ; http://n2t.net/addgene:19872 ; RRID:Addgene_19872)
For your References section:Molecular characterization of human Argonaute-containing ribonucleoprotein complexes and their bound target mRNAs. Landthaler M, Gaidatzis D, Rothballer A, Chen PY, Soll SJ, Dinic L, Ojo T, Hafner M, Zavolan M, Tuschl T. RNA. 2008 Oct 31. ():. 10.1261/rna.1351608 PubMed 18978028
Map uploaded by the depositor.