pNMSB99
(Plasmid #199308)

• Purpose: MosSCI insertion of myo-3p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChR2-HDRC::SL2::jRGECO1a::let-858 3'UTR at ttTi5605
• Depositing Lab: Michael Krieg
• Publication: Porta-de-la-Riva et al Nat Methods. 2023 Apr 6. doi: 10.1038/s41592-023-01836-9.

Backbone

• Vector backbone: pCFJ150
• Backbone size w/o insert (bp): 9131
• Total vector size (bp): 14474
• Vector type: Worm Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: myo-3p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChR2-HDRC::SL2::jRGECO1a::let-858 3'UTR
• Species: C. elegans (nematode)
• Insert Size (bp): 6707
• Mutation: mTagBFP2 from pJJR81, ChR2(H134R;D156C), C. elegans codon optimized jRGECO1a with 1 intron
• GenBank ID: OQ576354
• Promoter: myo-3

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: M13fwd
• 3′ sequencing primer: M13rev

Resource Information

• Supplemental Documents:
  • pnmsb99.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry