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(Plasmid #199551)


Item Catalog # Description Quantity Price (USD)
Plasmid 199551 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Vector type
    Mammalian Expression ; PiggyBac transposon vector
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Species
    H. sapiens (human)
  • Entrez Gene
    ONECUT1 (a.k.a. HNF-6, HNF6, HNF6A)
  • Entrez Gene
    HNF4A (a.k.a. FRTS4, HNF4, HNF4a7, HNF4a8, HNF4a9, HNF4alpha, MODY, MODY1, NR2A1, NR2A21, TCF, TCF-14, TCF14)
  • Entrez Gene
    HNF1B (a.k.a. ADTKD3, FJHN, HNF-1-beta, HNF-1B, HNF1beta, HNF2, HPC11, LF-B3, LFB3, MODY5, RCAD, T2D, TCF-2, TCF2, VHNF1)
  • Promoter TRE+CMVmin promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (not destroyed)
  • 3′ cloning site NheI (not destroyed)
  • 5′ sequencing primer CCCAGTCACGACGTTGTAAAACG
  • 3′ sequencing primer AGCGGATAACAATTTCACACAGG
  • (Common Sequencing Primers)

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PB513B-1/TRE-hHNF1β-hHNF4α-hHNF6-EF1α-Bla was a gift from Masamichi Kamihira (Addgene plasmid # 199551 ; ; RRID:Addgene_199551)
  • For your References section:

    HepG2-Based Designer Cells with Heat-Inducible Enhanced Liver Functions. Kitano H, Kawabe Y, Kamihira M. Cells. 2022 Apr 1;11(7):1194. doi: 10.3390/cells11071194. 10.3390/cells11071194 PubMed 35406758