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Depositing Lab
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Depositing OrganizationUniversity of Bristol
Located in United Kingdom of Great Britain and Northern Ireland -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 19974 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 19974-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 19974-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepTYF
- Backbone size w/o insert (bp) 7403
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGfaABC1D-EGFP
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SpeciesH. sapiens (human); jellyfish
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Insert Size (bp)2134
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer GTAGACATAA TAGCAACAGA C
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 19974-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 19974-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTYF-1xGfaABC1D-EGFP was a gift from Sergey Kasparov (Addgene plasmid # 19974 ; http://n2t.net/addgene:19974 ; RRID:Addgene_19974) -
For your References section:
Viral vectors based on bidirectional cell-specific mammalian promoters and transcriptional amplification strategy for use in vitro and in vivo. Liu B, Paton JF, Kasparov S. BMC Biotechnol. 2008 May 16;8:49. doi: 10.1186/1472-6750-8-49. 10.1186/1472-6750-8-49 PubMed 18485188
Map uploaded by the depositor.
