pAG306-pTet07-leuYFP[CTC]-nLuc
(Plasmid #199758)

• Purpose: Elongation reporter construct to quantify elongation of synYFP[CTC]
• Depositing Lab: Brian Zid
• Publication: Hou et al bioRxiv. 2023 Mar 20:2023.03.19.533377. doi: 10.1101/2023.03.19.533377. Preprint.

Backbone

• Vector backbone: pAG306
• Backbone manufacturer: Susan Lindquist
• Backbone size w/o insert (bp): 6656
• Vector type: Yeast Expression
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: leuYFP[CTC]
• Alt name: YFP[CTC]
• Species: Synthetic
• Insert Size (bp): 714
• Promoter: TetO7

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• A portion of this plasmid was derived from a plasmid made by: nanoLuc source: Anna et al. (https://doi.org/10.1002/yea.3155), synYFP[CTC] source: Subramaniam Lab

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • NanoLuc Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2023.03.19.533377v1 for bioRxiv preprint.