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HA-Elongin B-pcDNA3.1(+)-Zeo
(Plasmid #20000)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 20000 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1(+)-Zeo
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5022
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Bleocin (Zeocin)
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5alpha or XL1 @ 37˚C
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    transcription elongation factor B
  • Alt name
    Elongin B
  • Alt name
    TCEB2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    367
  • Mutation
    wild type
  • GenBank ID
    NM_007108
  • Entrez Gene
    ELOB (a.k.a. SIII, TCEB2)
  • Tag / Fusion Protein
    • HA-tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH I (not destroyed)
  • 3′ cloning site EcoR I (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HA-Elongin B-pcDNA3.1(+)-Zeo was a gift from William Kaelin (Addgene plasmid # 20000 ; http://n2t.net/addgene:20000 ; RRID:Addgene_20000)
  • For your References section:

    Ubiquitination of hypoxia-inducible factor requires direct binding to the beta-domain of the von Hippel-Lindau protein. Ohh M, Park CW, Ivan M, Hoffman MA, Kim TY, Huang LE, Pavletich N, Chau V, Kaelin WG. Nat Cell Biol. 2000 Jul . 2(7):423-7. 10.1038/35017054 PubMed 10878807