Atg20-24 pST39
(Plasmid #200439)

• Purpose: His6 tagged Atg20 and untagged Atg24 from S. cerevisiae for co-expression in E. coli.
• Depositing Lab: Michael Ragusa
• Publication: Reinhart et al Biochemistry. 2022 Apr 14. doi: 10.1021/acs.biochem.2c00007.

Backbone

• Vector backbone: pST39
• Total vector size (bp): 60093
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: Atg20
• Alt name: CVT20
• Alt name: SNX42
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 1977
• Tag / Fusion Protein:
  • 6xHis-TEV (N terminal on insert)

Gene/Insert 2

• Gene/Insert name: Atg24
• Alt name: SNX4
• Alt name: CVT13
• Insert Size (bp): 1275

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry