TS181_pETNKI_his3C_pA-Dam
(Plasmid
#200721)
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Purposeproducing pA-DamID methylation protein in bacteria
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 200721 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepET NKI
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameHis3C-pA-Dam
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Alt nameECO-Dam methylase
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Speciesbacteria
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Mutationcodon optimized
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Tag
/ Fusion Protein
- His (N terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
TS181_pETNKI_his3C_pA-Dam was a gift from Bas van Steensel (Addgene plasmid # 200721 ; http://n2t.net/addgene:200721 ; RRID:Addgene_200721) -
For your References section:
Genome-Wide Mapping and Microscopy Visualization of Protein-DNA Interactions by pA-DamID. van Schaik T, Manzo SG, van Steensel B. Methods Mol Biol. 2022;2458:215-229. doi: 10.1007/978-1-0716-2140-0_12. 10.1007/978-1-0716-2140-0_12 PubMed 35103970
Map uploaded by the depositor.
Map uploaded by the depositor.
