pND005
(Plasmid
#200951)
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Purpose(Empty Backbone) Yeast two-hybrid destination vector for Golden Gate cloning
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 200951 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneUnknown
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Backbone manufacturerUnknown
- Backbone size (bp) 6996
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Vector typeYeast Two-hybrid
- Promoter GAL4
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Selectable markersTRP1
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
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A portion of this plasmid was derived from a plasmid made byTimothy Whitehead Lab
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2023.04.13.536781v2 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pND005 was a gift from Timothy Whitehead (Addgene plasmid # 200951 ; http://n2t.net/addgene:200951 ; RRID:Addgene_200951) -
For your References section:
Standardizing cassette-based deep mutagenesis by Golden Gate assembly. Daffern N, Francino-Urdaniz IM, Baumer ZT, Whitehead TA. Biotechnol Bioeng. 2024 Jan;121(1):281-290. doi: 10.1002/bit.28564. Epub 2023 Sep 26. 10.1002/bit.28564 PubMed 37750676
Map uploaded by the depositor.
