pTn7-SCOUT21.RY
(Plasmid #201006)

• Purpose: pLv1-pUC18R6KT-miniTn7-Gm-mCherry-sYFP2, GmR, ApR
• Depositing Lab: Philip Poole
• Publication: Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10.1186/s40168-024-01792-2.

Backbone

• Vector backbone: pTn7-SCOUT21
• Backbone manufacturer: Philip Poole lab
• Backbone size w/o insert (bp): 5442
• Total vector size (bp): 6334
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin and Gentamicin, 100 & 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): EC100D pir+
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: mcherry
• Alt name: monomeric red fluorescent protein
• Species: Synthetic
• Insert Size (bp): 853
• GenBank ID: AAV52164
• Promoter: J23104

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: BpiI (destroyed during cloning)
• 3′ cloning site: BpiI (destroyed during cloning)
• 5′ sequencing primer: Golden Gate Assembly
• 3′ sequencing primer: Golden Gate Assembly

Gene/Insert 2

• Gene/Insert name: sYFP2
• Alt name: mVenus NB
• Species: Synthetic
• Insert Size (bp): 865
• GenBank ID: DQ092361.1
• Promoter: J23104

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: BpiI (destroyed during cloning)
• 3′ cloning site: BpiI (destroyed during cloning)
• 5′ sequencing primer: Golden Gate Assembly
• 3′ sequencing primer: Golden Gate Assembly

Resource Information

• Supplemental Documents:
  • pTn7-SCOUT21.RY.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Ec100D pir+ genotype:
F- mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL nupG pir+(DHRF) Maintains plasmids at ~15 copies per cell.
This strain is naturally resistant to streptomycin