BM28 ΔlysU
(Bacterial strain #201048)

• Purpose: MG1655-derived Escherichia coli isolate obtained from adaptive laboratory evolution to increasing incubation temperatures.
• Depositing Lab: Jeannette Winter
• Publication: McGuire et al BMC Genomics. 2023 Mar 27;24(1):154. doi: 10.1186/s12864-023-09266-9.

Backbone

• Vector backbone: the pOF39 plasmid backbone is pBR325

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): BM28 ΔlysU
• Growth instructions: Growth temperature: recommended 42-45 °C. Growth with kanamycin is not required as this marker is inserted in the chromosome. Grow on LB media as this strain was evolved in LB media. Grow at ≥42 °C to avoid reversion of thermotolerant phenotype. Please see accompanying papers for more information on the growth temperature range of the strain.
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please see the BM28 ΔlysU genome sequence (https://www.ncbi.nlm.nih.gov/nuccore/CP102379) and the accompanying papers for more details.

Additional publication:
Rudolph B, Gebendorfer KM, Buchner J, Winter J. Evolution of Escherichia coli for growth at high temperatures. J Biol Chem. 2010 Jun 18;285(25):19029-34. doi: 10.1074/jbc.M110.103374. Epub 2010 Apr 20. PMID: 20406805; PMCID: PMC2885180.