Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||20110||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5420
Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberLow Copy
Gene/Insert namep450 reductase and native Amorphadiene oxidase
Alt nameCPR and AMO
SpeciesC. tropicalis and A. annua
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pDUET-ctCPR-nAMO was a gift from Jay Keasling (Addgene plasmid # 20110 ; http://n2t.net/addgene:20110 ; RRID:Addgene_20110)
For your References section:Engineering Escherichia coli for production of functionalized terpenoids using plant P450s. Chang MC, Eachus RA, Trieu W, Ro DK, Keasling JD. Nat Chem Biol. 2007 May . 3(5):274-7. 10.1038/nchembio875 PubMed 17438551