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pPB-Neo-COVGT5-d2EGFP-mCherry
(Plasmid #201454)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 201454 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 201454-D50 50 μg of DNA in Tris buffer $495
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DNA 201454-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    Mammalian PiggyBac Expression Vector
  • Backbone manufacturer
    Vectorbuilder
  • Backbone size w/o insert (bp) 6931
  • Vector type
    Synthetic Biology
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    COVGT5_d2EGFP
  • Alt name
    COVGT5
  • Alt name
    COVGT4+COVGT1_d2EGFP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    8428

Cloning Information

  • Cloning method Unknown

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

We used SARS-CoV-2 signature & transcription factor genes to design reporters that model infection response in epithelial cells (Blanko-Melo et al.,2020; Wyler et al.,2020; Lamers et al.,2020).

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPB-Neo-COVGT5-d2EGFP-mCherry was a gift from Gaetano Gargiulo (Addgene plasmid # 201454 ; http://n2t.net/addgene:201454 ; RRID:Addgene_201454)

  • For your References section:

    Pharmacological modulators of epithelial immunity uncovered by synthetic genetic tracing of SARS-CoV-2 infection responses. Jiang B, Schmitt MJ, Rand U, Company C, Dramaretska Y, Grossmann M, Serresi M, Cicin-Sain L, Gargiulo G. Sci Adv. 2023 Jun 23;9(25):eadf4975. doi: 10.1126/sciadv.adf4975. Epub 2023 Jun 21. 10.1126/sciadv.adf4975 PubMed 37343108
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