pGTI25
(Plasmid #20170)

• Depositing Lab: Stephen West
• Publication: Tsaneva et al Mol Gen Genet. 1992 Oct . 235(1):1-10.

Backbone

• Vector backbone: pCP3
• Backbone manufacturer: n/a
• Backbone size w/o insert (bp): 7000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin and Kanamycin, 100 & 50 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): GT1265
• Growth instructions: Plasmid is supplied already transformed into strain GT1265 (ruvAB recA) containing plasmid c1857 (which encodes a temperature-sensitive lambda repressor. Grow at 30°C. Supplement media with 100µg/ml ampicillin and 30µg/ml kanamycin to maintain both plasmids.
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: RuvA
• Species: A. thaliana (mustard weed); E. coli
• Insert Size (bp): 609
• Mutation: none
• GenBank ID: BAA01034

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (destroyed during cloning)
• 3′ cloning site: BamHI (destroyed during cloning)
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Irina Tsaneva

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at or contact our distributors if you have any questions.