pET-His6-en15dFnCas9GFP
(Plasmid
#201951)
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PurposeExpression of en15dFnCas9-GFP in bacterial cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 201951 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepET-His6-GFP-TEV-LIC
- Backbone size w/o insert (bp) 6075
- Total vector size (bp) 11029
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Vector typeBacterial Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameExpression of en15dFnCas9-GFP in bacterial cells
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SpeciesSynthetic
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Insert Size (bp)4941
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MutationE1603H on FnCas9
- Promoter T7
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Tags
/ Fusion Proteins
- 6x His (N terminal on backbone)
- mEGFP (N terminal on backbone)
- HA (N terminal on insert)
- NLS (N terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer CAGCTCGCCGACCACTA (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET-His6-en15dFnCas9GFP was a gift from Debojyoti Chakraborty (Addgene plasmid # 201951 ; http://n2t.net/addgene:201951 ; RRID:Addgene_201951)