pMC-J6-dCas9-10XGP41-J9
(Plasmid
#202005)
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PurposeMoClo level 0 dCas9-10XGP41 coding sequence module
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Depositing Lab
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Depositing OrganizationUniversity of Minnesota
Located in United States of America -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 202005 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 202005-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 202005-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepJC221
- Backbone size w/o insert (bp) 2243
- Total vector size (bp) 7170
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Vector typeSynthetic Biology ; MoClo Cloning Vector
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namedCas9-10XGP41
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SpeciesSynthetic; Streptococcus pyogenes
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Insert Size (bp)4926
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Tag
/ Fusion Protein
- GS linkers
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 202005-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 202005-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMC-J6-dCas9-10XGP41-J9 was a gift from Michael Smanski (Addgene plasmid # 202005 ; http://n2t.net/addgene:202005 ; RRID:Addgene_202005) -
For your References section:
Efficient gene activation in plants by the MoonTag programmable transcriptional activator. Casas-Mollano JA, Zinselmeier MH, Sychla A, Smanski MJ. Nucleic Acids Res. 2023 Jul 21;51(13):7083-7093. doi: 10.1093/nar/gkad458. 10.1093/nar/gkad458 PubMed 37254802
Map uploaded by the depositor.
