pBIG2abc_hsHAUScomplex_FL
(Plasmid #202203)

• Purpose: Baculoviral transfer vector to co-express 8 subunits of human augmin complex
• Depositing Lab: Thomas Surrey
• Publication: Ustinova et al J Cell Sci. 2023 Jun 15;136(12):jcs261096. doi: 10.1242/jcs.261096. Epub 2023 Jun 26.

Backbone

• Vector backbone: pBIG2abc
• Backbone size w/o insert (bp): 5669
• Vector type: Insect Expression
• Selectable markers: Tetracycline

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: The depositor uses DH10EmBacY as the growing strain for the plasmid while transferring the plasmid to the Baculovirus system.
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: HAUS1
• Species: H. sapiens (human); codon-optimized form for expression in Trichoplusia ni cells
• GenBank ID: NM_138443.4
• Promoter: polyhedrin promoter
• Tag / Fusion Protein:
  • His-tag (6x) (C terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: aacgctctatggtctaaag
• 3′ sequencing primer: aaacgtgcaatagtatc

Gene/Insert 2

• Gene/Insert name: HAUS3
• Species: H. sapiens (human); codon-optimized form for expression in Trichoplusia ni cells
• GenBank ID: NM_001303143.2
• Promoter: polyhedrin promoter

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: AAACTGGATACTATTGCAC
• 3′ sequencing primer: aaccccgattgagatatag

Gene/Insert 3

• Gene/Insert name: HAUS2
• Species: H. sapiens (human); codon-optimized form for expression in Trichoplusia ni cells
• GenBank ID: NM_018097.3
• Promoter: polyhedrin promoter
• Tag / Fusion Protein:
  • -mGFP-TEV-TwinStrep (C terminal on insert)

Cloning Information for Gene/Insert 3

• Cloning method: Gibson Cloning
• 5′ sequencing primer: aacgctctatggtctaaag
• 3′ sequencing primer: aaacgtgcaatagtatc

Gene/Insert 4

• Gene/Insert name: HAUS6
• Species: H. sapiens (human); codon-optimized form for expression in Trichoplusia ni cells
• GenBank ID: NM_017645.5
• Promoter: polyhedrin promoter

Cloning Information for Gene/Insert 4

• Cloning method: Gibson Cloning
• 5′ sequencing primer: AAACTGGATACTATTGCAC
• 3′ sequencing primer: AAACATCAGGCATCATTAG

Gene/Insert 5

• Gene/Insert name: HAUS7
• Species: H. sapiens (human); codon-optimized form for expression in Trichoplusia ni cells
• GenBank ID: NM_001385482.1
• Promoter: polyhedrin promoter

Cloning Information for Gene/Insert 5

• Cloning method: Gibson Cloning
• 5′ sequencing primer: aaacctaatgatgcctg
• 3′ sequencing primer: aaccccgattgagatatag

Gene/Insert 6

• Gene/Insert name: HAUS4
• Species: H. sapiens (human); codon-optimized form for expression in Trichoplusia ni cells
• GenBank ID: NM_001166269.2
• Promoter: polyhedrin promoter

Cloning Information for Gene/Insert 6

• Cloning method: Gibson Cloning
• 5′ sequencing primer: aacgctctatggtctaaag
• 3′ sequencing primer: aaacgtgcaatagtatc

Gene/Insert 7

• Gene/Insert name: HAUS5
• Species: H. sapiens (human); codon-optimized form for expression in Trichoplusia ni cells
• GenBank ID: NM_015302.2
• Promoter: polyhedrin promoter

Cloning Information for Gene/Insert 7

• Cloning method: Gibson Cloning
• 5′ sequencing primer: AAACTGGATACTATTGCAC
• 3′ sequencing primer: AAACATCAGGCATCATTAG

Gene/Insert 8

• Gene/Insert name: HAUS8
• Species: H. sapiens (human); codon-optimized form for expression in Trichoplusia ni cells
• GenBank ID: NM_033417.2
• Promoter: polyhedrin promoter

Cloning Information for Gene/Insert 8

• Cloning method: Gibson Cloning
• 5′ sequencing primer: aaacctaatgatgcctg
• 3′ sequencing primer: aaccccgattgagatatag

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry