pBCC098
(Plasmid
#202224)
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PurposeLevel 2 empty backbone for strain-specific barcode DNA tag through Tn7 bacterial insertion, Kanamycin selectable marker and AmCyan1 fluorescent marker
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 202224 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepBCC34
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Vector typeBacterial Expression
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Selectable markersKanamycin
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Kanamycin, 25 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DB3.1λpir
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namepBCC25, pBCC32, pBCC56, pBCC27, pBCC26, pBCC28, pICH41822 (G5)
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SpeciesSynthetic
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2023.04.20.537712 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBCC098 was a gift from Paul Schulze-Lefert (Addgene plasmid # 202224 ; http://n2t.net/addgene:202224 ; RRID:Addgene_202224) -
For your References section:
Chromosomal barcodes for simultaneous tracking of near-isogenic bacterial strains in plant microbiota. Ordon J, Thouin J, Nakano RT, Ma KW, Zhang P, Huettel B, Garrido-Oter R, Schulze-Lefert P. Nat Microbiol. 2024 Apr;9(4):1117-1129. doi: 10.1038/s41564-024-01619-8. Epub 2024 Mar 19. 10.1038/s41564-024-01619-8 PubMed 38503974
