pBC160
(Plasmid #202248)

• Purpose: Level 2 for strain-specific barcode DNA tag with bc187 through Tn7 bacterial insertion, Gentamicin selectable marker and AmCyan1 fluorescent marker
• Depositing Lab: Paul Schulze-Lefert
• Publication: Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129. doi: 10.1038/s41564-024-01619-8. Epub 2024 Mar 19.

Backbone

• Vector backbone: pBCC087
• Vector type: Bacterial Expression
• Selectable markers: Gentamicin

Growth in Bacteria

• Bacterial Resistance(s): Kan + DAP
• Growth Temperature: 37°C
• Growth Strain(s): E.coli BW29427
• Growth instructions: Requires media that contains DAP. A supplement
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: bc187
• Species: Synthetic

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid can be transformed into E. coli BW29427 supplemented with 50 µg/mL of diaminopimelic acid (DAP) or any Pir1 strain. Please visit https://doi.org/10.1101/2023.04.20.537712 for bioRxiv preprint.