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Addgene

hTRAF2pLPCX-HA-Flag/P874
(Plasmid #20229)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 20229 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLPCX
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 6203
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    TNF receptor-associated factor 2
  • Alt name
    TRAF2
  • Alt name
    TRAP
  • Alt name
    TRAP3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1567
  • Mutation
    unmutated NM_021138.3. L replaces M at beginning of CDS after HA epitope.
  • GenBank ID
    NM_021138
  • Entrez Gene
    TRAF2 (a.k.a. MGC:45012, RNF117, TRAP, TRAP3)
  • Tags / Fusion Proteins
    • HA (N terminal on insert)
    • FLAG (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (destroyed during cloning)
  • 3′ cloning site ClaI (not destroyed)
  • 5′ sequencing primer pLNCX5' or CMV primer
  • 3′ sequencing primer pLNCX3'
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    ATTC
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hTRAF2pLPCX-HA-Flag/P874 was a gift from Martin Dorf (Addgene plasmid # 20229 ; http://n2t.net/addgene:20229 ; RRID:Addgene_20229)
  • For your References section:

    PKC phosphorylation of TRAF2 mediates IKKalpha/beta recruitment and K63-linked polyubiquitination. Li S, Wang L, Dorf ME. Mol Cell. 2009 Jan 16. 33(1):30-42. 10.1016/j.molcel.2008.11.023 PubMed 19150425