WTTEM1_pSTC0-zeo
(Plasmid
#202351)
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PurposeControl WT TEM-1 (wild type TEM-1 beta lactamase) in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette
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Depositing Lab
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Depositing OrganizationDana-Farber Cancer Institute
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 202351 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 202351-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 202351-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepSTC0-zeo
Growth in Bacteria
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Bacterial Resistance(s)Bleocin (Zeocin), 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameTEM-1
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SpeciesSynthetic
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Insert Size (bp)861
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2023.05.09.539914 for bioRxiv preprint.
DNA (Catalog # 202351-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 202351-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
WTTEM1_pSTC0-zeo was a gift from Nicholas Gauthier (Addgene plasmid # 202351 ; http://n2t.net/addgene:202351 ; RRID:Addgene_202351) -
For your References section:
Simultaneous enhancement of multiple functional properties using evolution-informed protein design. Fram B, Su Y, Truebridge I, Riesselman AJ, Ingraham JB, Passera A, Napier E, Thadani NN, Lim S, Roberts K, Kaur G, Stiffler MA, Marks DS, Bahl CD, Khan AR, Sander C, Gauthier NP. Nat Commun. 2024 Jun 20;15(1):5141. doi: 10.1038/s41467-024-49119-x. 10.1038/s41467-024-49119-x PubMed 38902262
Map uploaded by the depositor.
